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準備和存儲
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BD Pharmingen Human BD Fc Block 564219
BD Pharmingen™
Human (QC Testing), Rhesus (Tested in Development)
Human IgG1
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_2728082
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO
推薦的實驗流程
將 100 萬個細胞懸浮在 50-100 μL 染色緩沖液中與 2.5 μg 人 BD Fc Block™ 一起孵育（室溫下 10 分鐘），然后用所需的熒光抗體染色。封閉和染色步驟之間不需要洗滌步驟。
Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
BD Pharmingen Human BD Fc Block 564219
